Pre-Conference 3
1. Design of High-Dimensional Cytometry Panels
Co-facilitators: Cyrille MIONNET (CIML, Marseille, France) & Chris McRANDLE (SONY)
Overview:
The rapid evolution of cytometry instrumentation and reagents now enables fluorescence-based experiments with up to 50 markers, unlocking unprecedented biological insights. However, designing such high-dimensional panels can be daunting without a structured approach. This workshop is designed to empower participants with practical workflows to efficiently design panels ranging from 10 to 50 parameters on both conventional and spectral cytometers—minimizing wet-lab trial and error.
Workshop Objectives:
Through a combination of theoretical foundations and interactive discussions, this workshop will equip participants with the tools to:
- Master the basics: Understand the core principles of panel design, from fluorochrome selection to instrument compatibility;
- Navigate common pitfalls: Identify and address typical challenges in high-dimensional panel design;
- Optimize panel performance: Learn to balance sensitivity, resolution, and data quality.
Key Topics:
- Spillover Spreading Error (SE): Why it matters and how to mitigate its impact on panel design.
- Similarity and Complexity Index: Strengths, limitations, and practical applications.
- Spectral Cytometry Challenges: Introduction to a novel type of spreading error specific to spectral platforms.
- Step-by-Step Workflow: A comprehensive, platform-agnostic guide to designing and troubleshooting polychromatic panels.
Interactive Format: Participants will engage in real-time discussions and case studies to apply the concepts covered. Bring your questions and challenges—this workshop is tailored to address your specific needs!
Who Should Attend?
- Researchers, technicians, and students working with conventional or spectral cytometry.
- Anyone looking to expand their panel design skills or optimize existing workflows.
Outcome: By the end of this workshop, participants will have the knowledge, tools, and confidence to design and troubleshoot high-dimensional cytometry panels—regardless of the platform.