SFI-AFC 2022

Rhodes - Level 2 Meeting registration

Auditorium Athéna - Level 2Welcome introduction

• Renato Monteiro, President of the SFI
• Catherine Blanc, President of the AFC

Auditorium Athéna - Level 2New concepts in immunology

Chairs: Abdelilah Wakkach (Nice), Aïda Meghraoui-Kheddar (Biot) & Hervé Luche (Marseille)

• Yasmine Belkaid (Bethesda, USA): Microbiome control of host immunity

Rhodes - Level 2Coffee break - Visit of exhibition - Posters

Auditorium Athéna - Level 2

High content imaging

Chairs: Aïda Meghraoui-Kheddar (Biot) & Noel De Miranda (The Netherlands)

• Laura Kuett (Switzerland): 3D imaging mass cytometry for cell-level mapping of tissues
• Noel De Miranda (The Netherlands): The many faces of colorectal cancer immunity revealed by high content imaging approaches

Selected short oral presentations

• Meri Tulic (Nice): Unraveling the Complexity of the Tissue Microenvironment in the Skin of Vitiligo Patients using High-Plex Spatial Imaging
• Soizic Garaud (Brest): Dysregulation of B-cell homeostasis at the onset of immune checkpoint inhibitor toxicity in cancer patients
• Christophe Goncalves (Montreal, Canada): Highly Multiplexed Phenotyping of the Immune Tumor Microenvironment by CODEX Tissue Imaging
• Yaël Glasson (Montpellier): A 31-plex panel for high-dimensional single cell analysis of murine preclinical models of solid tumors by imaging mass cytometry

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Room Gallieni 5 - Level 2

Osteoimmunology

Chairs: Claudine Blin (Nice) & Mario Zaiss (Erlangen, Germany)

• Claudine Blin (Nice): Diversity and immune functions of osteoclasts, the multinucleated myeloid cells
• Thibault Griseri (Londres, UK): Dysregulated hematopoiesis in spondyloarthritis

Selected short oral presentations

• Julia Halper (Nice): Transcriptomic mapping of osteoclasts highlights PRRs in pathological osteoclastogenesis
• Simon Glatigny (Montigny-le-bretonnneux): Pathogenicity of IL-17 producing cells during Spondyloarthritis

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Room Rhodes 9 - Level 2

Innate lymphoid cells

Chairs: Émilie Narni-Mancinelli (Marseille) & Matthieu Rouleau (Nice)

• Dirk Elewaut (Ghent, Belgium): Innate like T cells and arthritis: gateway from gut to joints
• Emilie Narni-Mancinelli (Marseille): ILC in health and disease

Selected short oral presentations

• Clémence Riffard (Paris): Acute and chronic inflammation of pulmonary tissue provokes an increase in LTi-like ILC3 that parallels TLS neogenesis
• Loïs Coënon (Montpellier): Non-genetically modified, CAR-like, NK cell

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Room Risso 6 - Level 2

AFC / CytHem - Myeloma

Chairs: Jérôme Moreaux (Montpellier) & Thomas Matthes (Geneva, Switzerland)

• Thomas Boyer (Amiens): L’Intelligence Artificielle associée à la Cytométrie en Flux comme aide à la classification des Dyscrasies Plasmocytaires
• Andreas Beilhack (Würzburg, Germany): Targeting the multiple myeloma microenvironment
• Jérôme Moreaux (Montpellier): Predictive markers of response to Daratumumab in Multiple Myeloma

Selected short oral presentations

• Mohamad Cherry (Saint-Etienne): Myeloid lineage evaluation in patients with suspected myeloma
• Hélène Gardeney (Poitiers): Innate T-cells in the multiple myeloma tumoral niche

Rhodes - Level 2 Lunch

Coffee - Visit of exhibition - Posters

Room Risso 6 - Level 2

La maladie résiduelle par cytométrie en flux dans les leucémies aiguës myéloblastiques

Modérateurs : Adriana Plesa (Lyon) & Christophe Roumier (Lille)

• Wolfgang Kern (Munich, Allemagne): Flow cytometric assessment of MRD in AML
• Claude Preudhomme (Lille): MRD moleculaire/NGS
• Thomas Cluzeau (Nice): MRD AML et aspect clinique

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Room Gallieni 5 - Level 2

SFI Club: Mast Cells

Chairs: Nicolas Gaudenzio (Toulouse) & Laurent Reber (Toulouse)

• Florent Ginhoux (Villejuif): New insights in the role of fetal mast cells in the onset of early life allergies
• Nicolas Gaudenzio (Toulouse): Single cell analysis of mast cells across organs: from development to heterogeneity
• Laurent Reber (Toulouse): Development of an anti-IgE vaccine for the treatment of IgE- and mast cell/basophil-mediated diseases

Selected short oral presentation

• Jasper Kamphuis (Toulouse): Impact of ageing on the severity of IgE-, Mrgprb2-, and histamine-mediated anaphylaxis in mice

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Auditorium Athéna - Level 2

SFI Club: Neuroimmunology

Chairs: Lennart Mars (Lille) & Violetta Zujovic (Paris)

• Henrique Veiga-Fernandes (Portugal): Neuronal regulation of innate lymphoid cells
• Sophie Ugolini (Marseille): Neuroimmune interactions in the skin, from molecular mechanisms to therapeutic perspectives
• Séverine Boillée (Paris): Peripheral macrophages and microglial cells for the progression of ALS

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Room Rhodes 9 - Level 2

SFI Club: Germinal Center

Chairs: Nicolas Fazilleau (Toulouse) & Patricia Amé-Thomas (Rennes)

• Mauro Gaya (Marseille): B cell memory in the lung airways
• Christopher Mueller (Strasbourg): Formation of the B cell-associated stromal cells in the lymph node

Selected short oral presentations

• Helena Paidassi (Lyon): Fine-tuning germinal centre B cell responses via αvβ8-mediated activation of TGFβ
• Pascal Chappert (Paris): Human anti-Smallpox long-lived memory B cells are defined by dynamic interactions in the splenic niche and long-lasting germinal center imprinting

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Room Risso 6 - Level 2

Eco-responsible approach in the laboratory

Chair: Muriel Andrieu (Paris)

• Peggy Sanatine (Évry): Biosécurité et environnement : Évolution
• Emmanuelle Näser (Toulouse): Biosécurité et environnement : Retour du questionnaire déchets

Auditorium Athéna - Level 2Industry talk

Chairs: Florent Raffin (Brétrigny-sur-Orge) & Hans Yssel (Paris)

• BioLegend
  Explore our latest reagents for multiparametric flow cytometry
  Élodie Baudoin (BioLegend France SAS)

• NanoString
  Catalyze The Next Revolution Using Spatial Biology: Three Platforms. Unlimited Potential
  Thibaud Metzger (NanoString)

• Agilent Technologies
  Agilent: The Cell Analysis Solution
  Jérome Darakdjian (Agilent) & Damien Brechet (BioTek Instruments France)

• Thermo Fisher Scientific
  La légende du BigFoot
  Guerric Epron (Thermo Fisher Scientific)

• Stemcell Technologies
  Fast and Easy Immune Cell Isolation from Large-Volume Samples
  Sonika Godavarthy (Stemcell Technologies)

• Standard BioTools
  Ready-to-use 45+ flow cytometry marker panels to measure activation, cytokine production and cytotoxicity from < 300ul of whole blood
  Sathiesan Thambirajah (Standard BioTools)

• Bio-Rad
  Build Bigger Better Panels with StarBright Dyes
  Andrea Tradori (Bio-Rad Laboratories)

• Vizgen
  Explore new dimensions through spatial context
  Mickael Ploquin (EMEA - Vizgen)

• Merck
  The latest Antibody innovations from Merck: ZooMAb^® recombinant monoclonal antibodies and ColorWheel^® Flow Cytometry Antibodies & Dyes
  Baptiste Ménard (Protein Biology Research)

Room Risso 6 - Level 2

Suivi biologique des immunothérapies

Modératrices : Morgane Gossez-Coulaud (Lyon) & Guislaine Carcelain (Paris)

•  Aude Gleizes (Paris) : Anti-TNFα
• Marie-Agnès Dragon-Durey (Paris) : Anti-checkpoints inhibiteurs
• Rémi Pescarmona (Lyon) : Transfert de cellules CAR-T
• Sophie Candon (Rouen) : Anti-CD20

Rhodes - Level 2 Poster Session 1 - odd-numbered posters

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Rhodes - Level 2 Coffee break - Visit of exhibition

Auditorium Athéna - Level 2Industry Symposium

Chairs: Catherine Blanc (Paris) & Patricia Amé-Thomas (Rennes)

• Beckman Coulter Life Sciences
  IVDR - Perspective d’un fabricant : Partenariat pour une transition en douceur
  Brice Ezzouaouy (Immunotech Marseille)

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Auditorium Athéna - Level 2Industry talk

Chairs: Catherine Blanc (Paris) & Patricia Amé-Thomas (Rennes)

• 10x Genomics
  Hypothesis-free, multi modal analysis of the immune landscape at single cell and spatial resolution
  Christophe Fleury (10x Genomics)

• BD
  BD FACSDiscover™ S8 et la technique d’imagerie BD CellView™ - Le premier cytomètre trieur combinant l’analyse spectrale et l’analyse d’image
  Thierry Langlois (BD)

Room Risso 6 - Level 2

Core facility session: ‘Standardisation: from tissue to sorted cells’

Chairs: Julie Cazareth (Valbonne) & Alexia Zakaroff (Toulouse)

• Alexia Zakaroff (Toulouse): Good practices for standardization: from sample to instrument
• Pierre Grenot (Strasbourg): Les bonnes pratiques de standardisation : de l’instrument à l’analyse

Selected short oral presentation

• Carolina Moraes Cabé (Paris): CB UTechS, Institut Pasteur: a successful model of core facility for integrated and project-tailored support for biomarker discovery in immunology
• Coralie Guerin (Paris): Single-cell resolution tissue cytometry: a new multiplex spatial spectral approach

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Room Rhodes 9 - Level 2

Myeloid cells

Chairs: Stoyan Ivanov (Nice) & Martin Guilliams (Belgium)

• David Sancho (Madrid, Spain): Oxidative phosphorylation selectively orchestrates tissue macrophage homeostasis
• Florent Ginhoux (Paris): Myeloid Cell Heterogeneity in Health and Disease

Selected short oral presentations

• Ignacio Cebrián (Barcelona, Spain): Dendritic cell phagosomes recruit GRASP55 for efficient transport of MHC molecules to the plasma membrane during exogenous antigen presentation
• Despoina Koumantou (Paris): The p84/p110γ complex of PI3Kγ controls FcγR-inducible and antibody-dependent cross-presentation in type 2 DCs and GM-CSF derived inflammatory DCs
• Raquel Sal Carro (Strasbourg): Epidermal maintenance of Langerhans cells relies on autophagy-regulated lipid metabolism

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Auditorium Athéna - Level 2

Host/pathogen Interactions

Chairs: Christophe Paget (Tours) & Marlène Dreux (Lyon)

• Olivier Neyrolles (Toulouse): C-type lectins in immunity to tuberculosis and beyond
• Marlène Dreux (Lyon): Severe COVID-19 patients have impaired plasmacytoid dendritic cell-mediated control of SARS-CoV-2-infected cells

Selected short oral presentations

• Jane Jardine (Nantes): The counterposing roles played by CK1a fine-tune the cGAS-STING pathway
• Benjamin Voisin (Strasbourg): An innervated and immunocompetent human skin model to study arboviral cutaneous infection
• Emmanuel Martin (Paris): Dual role of IL-27 in immunity to Epstein-Barr virus infection revealed by IL27RA deficiency

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Room Gallieni 5 - Level 2

AFC / CytHem - AML & MDS

Chairs: Nicolas Chapuis (Paris) & François Vergez (Toulouse)

• Rafaël Argüello (Marseille): Metabolic and epigenetic profiling with single cell resolution in a fixation-compatible system using whole blood samples
• Nicolas Dulphy (Paris): Intrinsic and Extrinsic Defects for Natural Killer cell-mediated Immunosurveillance in Myelodysplastic Syndromes

Selected short oral presentations

• Rawan Hallal (Montpellier): Targeting the SUMO pathway activates Natural Killer cells and enhances their anti-tumoral activity in Acute myeloid leukemia
• Thomas Matthes (Geneva, Switzerland): In-depth analysis of CD34pos hematopoietic stem and precursor cell (HSPC) subpopulations by flow cytometry using a single 20-color panel
• Carmen-Mariana Aanei (Saint-Etienne): High-dimensional mass cytometry analysis of the leukemic cells and of their signaling pathways in AML